国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (11): 862-865.doi: 10.3760/cma.j.issn.1673-422X.2014.11.019

• 论著 • 上一篇    下一篇

斑蝥酸钠对体外培养肺癌患者淋巴细胞免疫应答的影响

  

  1. 河北省保定市第一中心医院胸外科（邢震宇、张会弟、高磊），检验科（常文龙），手术室（张辉）；保定市第三医院神经外科（田少辉）
  • 出版日期:2014-12-03 发布日期:2015-01-20
  • 通讯作者: 邢震宇，Email：cherokeeman@sohu.com
  • 基金资助:

    河北省卫生厅青年科技课题(20130018)

Effect of disodium cantharidinate on in vitro lymphocyte immune response in lung cancer patients

  1. Department of Chest Surgery, First Central Hospital of Baoding, Baoding 071000, China
  • Online:2014-12-03 Published:2015-01-20
  • Contact: Xing Zhenyu, Email: cherokeeman@sohu.com

PDF

1296

被引次数

2

摘要： 目的探讨斑蝥酸钠对体外培养肺癌患者淋巴细胞免疫应答的影响。方法病理诊断为非小细胞肺癌的患者20例，体外进行人外周血淋巴细胞培养，应用四甲基偶氮唑盐法检测不同浓度斑蝥酸钠维生素B6作用下淋巴细胞的增殖影响情况，应用流式细胞仪分析不同浓度斑蝥酸钠对CD4+ CD25+ T细胞、CD25+FOXP3+ T细胞、CD4+和CD8+T细胞的影响。结果当斑蝥酸钠维生素B6注射液浓度＜10 μg/ml时，浓度升高能刺激外周血淋巴细胞的增殖。斑蝥酸钠维生素B6浓度＞10μg/ml，随着浓度的升高，斑蝥酸钠对淋巴细胞增殖的刺激作用减弱。当斑蝥酸钠维生素B6注射液浓度＜10 μg/ml时，随着实验组浓度的升高，与对照组相比，CD4+/CD8+比值明显升高（t=2.171，P=0.032；t=2.103，P=0.041；t=3.662，P=0.002；t=3.201，P=0.003；t=3.233，P=0.003)，CD4+ CD25+/CD4+和CD25+ FOXP3+T明显降低，差异有统计学意义；但当斑蝥酸钠维生素B6注射液浓度高于10 μg/ml时,斑蝥酸钠10、15、20 μg/ml组CD4+/CD8+ 比值、CD4+ CD25+/CD4+和CD25+ FOXP3+T变化均不大。结论斑蝥酸钠维生素B6在一定范围内能促进体外培养肺癌患者淋巴细胞的增殖，并提高其细胞免疫能力。

关键词: 肺肿瘤, 细胞培养技术, 自身免疫, 斑蝥酸钠

Abstract: Effect of disodium cantharidinate on in vitro lymphocyte immune response in lung cancer patientsXing Zhenyu*, Zhang Huidi, Gao Lei, Chang Wenlong, Tian Shaohui, Zhang Hui. *Corresponding author: 【Abstract】ObjectiveTo investigate the effect of disodium cantharidinateon in vitro lymphocyte immune response in lung cancer patients. MethodsTwenty nonsmall cell lung cancer patients diagnosed by pathological diagnosis were selected. The effects of different concentrations of disodium cantharidinate and vitamin B6 on lymphocyte proliferation and CD4+CD25+ T cells, CD25+FOXP3+ T cells, CD4+ and CD8+ T cells were detected by methyl thiazolyl tetrazolium assay and flow cytometry. ResultsWhen the concentrations of disodium cantharidinate and vitamin B6 was less than 10 μg/ml, with the growth of concentration, it stimulated the proliferation of peripheral blood lymphocytes. When the concentration was more than 10 μg/ml, with the growth of concentration, the absorbance value decreased, and the stimulating effect weakened. When the concentration of disodium cantharidinate and vitamin B6 injection was less than 10 μg/ml, with the growth of concentration, CD4+/CD8+ ratio increased significantly, and CD4+ CD25+/CD4+ and CD25+ FOXP3+ T cells were significantly lower, which showed statistically significant differences （t=2.171, P=0.032; t=2.103, P=0.041; t=3.662, P=0.002; t=3.201, P=0.003; t=3.233, P=0.003). But when the concentration was more than 10 μg/ml, The differences of CD4+/CD8+ ratio, CD4+CD25+/CD4+ and CD25+FOXP3+ T cells in 10, 15, 20 μg/ml groups were not statistically significant, which showed that disodium cantharidinate and vitamin B6 injection had a positive effect on enhancing the immune function of lymphocytes in lung cancer in a concentration dependent manner, but high concentration was unhelpful. ConclusionDisodium cantharidinate and vitamin B6 injection can promote lung cancer in vitro lymphocyte proliferation as well as increase its immunity in a certain range.

Key words: Lung neoplasms, Cell culture techniques, Autoimmunity, Disodium cantharidinate