国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (8): 688-692.doi: 10.3760/cma.j.issn.1673-422X.2014.09.014

• 论著 • 上一篇    下一篇

非小细胞肺癌中DLC-1、ROCKⅠ的表达及其 临床意义

翟玉洁,范庆帅,宁方玲,刘长民,赵大华,陈绍水   

  1. 256600 滨州医学院附属医院肿瘤科
  • 出版日期:2014-09-25 发布日期:2014-09-05
  • 通讯作者: 陈绍水 E-mail:chenshaoshui@yeah.net

Expressions and clinical significances of DLC-1 and ROCKⅠ in nonsmall cell lung cancer

Zhai Yujie, Fan Qingshuai, Ning Fangling, Liu Changmin, Zhao Dahua, Chen Shaoshui   

  1. Department of Oncology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China
  • Online:2014-09-25 Published:2014-09-05
  • Contact: Chen Shaoshui E-mail:chenshaoshui@yeah.net

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摘要： 目的探讨肝癌缺失基因1（DLC1）和Rho相关卷曲螺旋形成蛋白激酶Ⅰ(ROCKⅠ)在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法选取48例手术切除并经病理检查证实的NSCLC组织及癌旁组织，采用免疫组织化学EnVision法检测DLC1、ROCKⅠ蛋白的表达，并分析二者之间的相关性及其与临床病理特征的关系，探讨DLC1基因对NSCLC患者的预后价值。结果DLC1蛋白在NSCLC组织中呈低表达或表达缺失，阳性表达率为33.3%（16/48），明显低于癌旁组织的70.8%（34/48），差异有统计学意义（χ2=13.523，P＜0.01）；ROCKⅠ蛋白在NSCLC中的阳性表达率为58.3%（28/48），高于癌旁组织的0（0/48），差异有统计学意义(χ2=39.529，P＜0.01)；NSCLC中DLC1的表达与性别、吸烟史、组织类型等无关，与肿瘤分化程度、淋巴结转移、TNM分期有关，且差异有统计学意义；DLC1阳性表达组中ROCKⅠ的阳性表达率为37.5%（6/16），而ROCKⅠ的阴性表达率为68.8%（22/32），相关分析提示DLC1与ROCKⅠ在NSCLC中表达呈负相关（r=-2.214，P=0.039）；DLC1蛋白高表达组3年生存率高于低表达组，差异有统计学意义（P=0.043）。结论DLC1蛋白的低表达或表达缺失、ROCKⅠ蛋白的表达在NSCLC的发生、发展中可能扮演重要角色，检测DLC1及ROCKⅠ蛋白的表达，有助于判断NSCLC的生物学行为及预后。

关键词: 癌, 非小细胞肺, 预后, 肝癌缺失基因-1, Rho相关卷曲螺旋形成蛋白酶Ⅰ

Abstract: ObjectiveTo explore the expressions and clinical significances of deleted in liver cancer1 (DLC1) and Rho associated coiledcoil forming protein kinase (ROCK)Ⅰ in nonsmall lung cancer (NSCLC). MethodsThe expressions of DLC1 and ROCKⅠ in NSCLC and adjacent tissue of 48 patients with pathologically confirmed as NSCLC and undergone surgical resection were detected by immunohistochemistry EnVision method. The correlations among DLC1 protein, ROCKⅠ protein and the clinical pathological characteristics were analyzed. The prognostic value of DLC1 in patients with NSCLC was studied. ResultsThe expression of DLC1 protein in NSCLC tissue was low or missing, and the positive rate was 33.3% (16/48), significantly lower than that in the tissue adjacent to carcinoma 70.8% (34/48), with statistical significance (χ2=13.523, P<0.01). The positive expression rate of ROCKⅠ protein in NSCLC was 58.3% (28/48), higher than that of tissue adjacent to carcinoma 0（0/48）, with statistical significance (χ2=39.529, P<0.01). The expression of DLC1 protein was correlated with tumor differentiation, lymph node metastasis and clinical stage, rather than with sex, smoking history and organization type. Through the correlation analysis, the expression of ROCKⅠ in DLC1 positive group was 37.5% (6/16), and the expression rate of ROCKⅠ in DLC1 negative group was 68.8% (22/32). There was negative correlation between DLC1 and ROCKⅠ in NSCLC tissues (r=-2.214, P=0.039). The 3 year survival rate in DLC1 protein high expression group was obviously higher than that in low expression group, with statistical significance (P=0.043). ConclusionLow or missing expression of DLC1 and high expression of ROCKⅠ protein may play an important role in the occurrence and development of NSCLC. Detecting the expression of DLC1 and ROCKⅠ protein may be useful for evaluating the biological behavior and prognosis of NSCLC.

Key words: Carcinoma, nonsmallcell lung, Prognosis, DLC-1, ROCKⅠ