Clinical value of serum miR-19b and miR-744-5p levels in the diagnosis of non-small cell lung cancer

doi:10.3760/cma.j.cn371439-20230308-00011

Abstract

Abstract:

Objective To investigate the serum levels of miR-19b and miR-744-5p in patients with non-small cell lung cancer （NSCLC），and to analyze the clinical value of miR-19b and miR-744-5p in the diagnosis of NSCLC. Methods A total of 226 NSCLC patients （NSCLC group）and 100 healthy people （control group）admitted to Jilin Cancer Hospital from August 2019 to August 2022 were selected as research objects. Quantitative real-time PCR was used to measure and compare the serum levels of miR-19b and miR-744-5p between the NSCLC group and the control group，and the relationships between the two indicators and different clinical and pathological characteristics of NSCLC patients were analyzed. The receiver operating characteristic curve was used to analyze the clinical value of miR-19b，miR-744-5p and their joint detection in the diagnosis of NSCLC. Results Compared with the control group，the serum miR-19b level （3.86±1.25 vs. 1.06±0.41）in the NSCLC group significantly increased （t=21.87，P<0.001），while the miR-744-5p level （1.80±0.48 vs. 5.75±1.69）significantly decreased （t=32.36，P<0.001）. The serum miR-19b levels in NSCLC patients with pathological types of adenocarcinoma，maximum tumor diameter ≥3 cm，medium to low differentiation，stage Ⅲ-Ⅳ，and with lymph node metastasis were higher than those in squamous cell carcinoma （t=5.94，P<0.001），maximum tumor diameter <3 cm （t=2.65，P=0.009），well differentiation （t=4.33，P<0.001），stageⅠ-Ⅱ （t=12.32，P<0.001），patients without lymph node metastasis （t=8.13，P<0.001），while miR-744-5p levels were lower than those in squamous cell carcinoma （t=8.27，P<0.001），tumor maximum diameter <3 cm （t=5.34，P<0.001），well differentiation （t=6.95，P<0.001），stageⅠ-Ⅱ （t=11.40，P<0.001），patients without lymph node metastasis （t=10.36，P<0.001）. The area under the curve （AUC）of serum miR-19b combined with miR-744-5p in the diagnosis of NSCLC was 0.914 （95%CI：0.841-0.959），with sensitivity and specificity of 90.9% and 84.0%，respectively. AUC was significantly than that of the single indicator detection of miR-19b （AUC=0.824，95%CI：0.770-0.869）and miR-744-5p （AUC=0.783，95%CI：0.709-0.838）（Z=2.28，P=0.021； Z=2.36，P=0.017）. Conclusion Serum miR-19b level of NSCLC patients is increased，miR-744-5p levels is decreased，and joint detection of serum miR-19b and miR-744-5p has high clinical value in the diagnosis of NSCLC.

Key words: Carcinoma, non-small-cell lung, miR-19b, miR-744-5p, Serum tumor markers

Li Dan, Li Ruiyao, Li Yinghan, Yu Xiuyan, Wu Xuefeng. Clinical value of serum miR-19b and miR-744-5p levels in the diagnosis of non-small cell lung cancer[J]. Journal of International Oncology, 2024, 51(2): 83-88.

Figures/Tables 6

References 19

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基因	正向引物（5'-3'）	反向引物（5'-3'）
miR-19b	UGUGCAAAUCCAUGCAAAACUGA	GCTCACTGCAACCTCCTCCTCC
miR-744-5p	AAGGTGCGGGGCTAGGGCTAA	AGTAAGGTTGAGGTTA
U6	GCTTCGGCAGCACATATACTAAAT	CGCTTCACGAATTTGCGTGTACT

临床病理特征	例数		miR-19b		t值		P值	miR-744-5p		t值	P值
性别
男	139		4.14±1.33		1.95		0.052	5.88±1.76		1.40	0.163
女	87		3.79±1.28		1.95		0.052	5.54±1.80		1.40	0.163
年龄（岁）
≥60	154		4.14±1.39		1.73		0.086	5.93±1.79		1.90	0.059
<60	72		3.81±1.22		1.73		0.086	5.46±1.61		1.90	0.059
吸烟
是	57		4.11±1.35		1.74		0.083	5.98±1.82		1.79	0.075
否	169		3.76±1.30		1.74		0.083	5.51±1.68		1.79	0.075
肿瘤位置
右肺	128		3.92±1.22		0.81		0.422	5.82±1.67		0.88	0.381
左肺	98		3.79±1.18		0.81		0.422	5.62±1.73		0.88	0.381
病理类型
腺癌	141	4.52±1.34		5.94		<0.001		4.82±1.56	8.27		<0.001
鳞状细胞癌	85	3.48±1.16		5.94		<0.001		6.67±1.74	8.27		<0.001
肿瘤最大径（cm）
<3	144	3.56±1.09		2.65		0.009		6.36±1.62	5.34		<0.001
≥3	82	3.98±1.24		2.65		0.009		5.15±1.67	5.34		<0.001
分化程度
中低分化	136	4.23±1.35		4.33		<0.001		4.98±1.52	6.95		<0.001
高分化	90	3.46±1.13		4.33		<0.001		6.54±1.73	6.95		<0.001
TNM分期
Ⅰ~Ⅱ期	155	2.83±1.04		12.32		<0.001		7.35±2.08	11.40		<0.001
Ⅲ~Ⅳ期	71	4.96±1.51		12.32		<0.001		4.29±1.31	11.40		<0.001
淋巴转移
有	68	4.64±1.34		8.13		<0.001		4.48±1.33	10.36		<0.001
无	158	3.26±1.09		8.13		<0.001		7.27±2.04	10.36		<0.001