国际肿瘤学杂志

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (5): 340-342.doi: 10.3760/cma.j.issn.1673-422X.2016.05.005

• 论著 • 上一篇    下一篇

非小细胞肺癌ALK基因重排检测

赵小磊,李三华,刘玲玲,梁永波,牛银银,翟晋豫,徐玉茵,邱金奎,齐华   

  1. 450001 郑州,河南省食品药品审评查验中心（赵小磊）；郑州市病理诊断技术工程研究中心 河南赛诺特生物技术有限公司研发中心（李三华、刘玲玲、梁永波、牛银银、翟晋豫、邱金奎、齐华）；河南省医疗器械检验所（徐玉茵）
  • 出版日期:2016-05-08 发布日期:2016-04-07
  • 通讯作者: 齐华 E-mail:qihua63@163.com
  • 基金资助:

    郑州市科技重大专项(152PZDZX029)

Detection of ALK gene rearrangement in nonsmall cell lung cancer

Zhao Xiaolei, Li Sanhua, Liu Lingling, Liang Yongbo, Niu Yinyin, Zhai Jinyu, Xu Yuyin, Qiu Jinkui, Qi Hua   

  1. Henan Food and Drug Evaluation Identification Center, Zhengzhou 450001, China
  • Online:2016-05-08 Published:2016-04-07
  • Contact: Qi Hua E-mail:qihua63@163.com
  • Supported by:

    Science and Technology Major Project of Zhengzhou (152PZDZX029)

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摘要： 目的通过荧光原位杂交（FISH）方法检测非小细胞肺癌(NSCLC)患者间变性淋巴瘤激酶（ALK）基因重排。方法根据ALK断裂重排方式及特点，设计并制备红/绿双色荧光探针。以人外周血培养细胞为检测对象评价ALK 融合基因检测探针的敏感性和特异性，以NSCLC石蜡组织样本为对象进行ALK基因重排检测以评价探针的性能。结果本研究制备的荧光探针在EB病毒（EBV）转化的人淋巴细胞检测中的特异性和敏感性均可达到100%。在对2例NSCLC患者石蜡包埋组织样本检测中，检测阴性、阳性各1例，检测结果与免疫组织化学检测结果一致。结论本研究中制备的双色荧光探针可用于NSCLC ALK基因重排的检测。

关键词: 癌, 非小细胞肺, 原位杂交, 荧光, ALK基因重排

Abstract: ObjectiveTo detect  anaplastic lymphoma kinase (ALK) gene rearrangement in nonsmall cell lung cancer (NSCLC) by fluorescent in situ hybridization (FISH). MethodsAccording to ALK gene rearrangement manners, dualcolor (Red/Green) fluorescence probes were designed and labeled. Specificity and sensitivity were evaluated in peripheral blood metaphase of lymphocyte cells. Test performances were evaluated in NSCLC paraffin tissue samples by FISH and immunohistochemistry. ResultsBoth specificity and sensitivity reached 100% in EpsteinBarr virus (EBV) transformed human lymphocyte cells. The established FISH method detected one ALK gene rearrangement case among two NSCLC samples successfully. The results of FISH were consistent with the results of immunohistochemistry. ConclusionThe dualcolor probe designed in this study can be used for ALK gene rearrangement testing in NSCLC.

Key words: Carcinoma, nonsmallcell lung, In situ hybridization, fluorescence, ALK gene rearrangement