国际肿瘤学杂志

国际肿瘤学杂志 ›› 2012, Vol. 39 ›› Issue (4): 316-320.

• 论著 • 上一篇    

大黄素诱导胆管癌QBC939细胞凋亡的实验研究

李新星, 王坚   

  1. 200127 上海交通大学医学院附属仁济医院普外科
  • 出版日期:2012-04-08 发布日期:2012-03-28
  • 通讯作者: 王坚，E-mail: dr_wangjian@yahoo.com.cn E-mail:dr_wangjian@yahoo.com.cn
  • 基金资助:

    上海市科学技术委员会科研计划项目（09411960800）

Experimental study of emodin inducing apoptosis of bile duct carcinoma cell QBC939

LI  Xin-Xing, WANG  Jian   

  1. Department of General Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2012-04-08 Published:2012-03-28
  • Contact: Corresponding author: WANG Jian, E-mail: dr_wangjian@yahoo.com.cn E-mail:dr_wangjian@yahoo.com.cn

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摘要： 目的 探讨大黄素在体外诱导人胆管癌QBC939细胞凋亡的作用及机制。方法 采用3-(4，5-二甲基噻唑-2)-2，5-二苯基四氮唑溴盐(MTT)法检测大黄素对细胞增殖的抑制作用；在荧光显微镜下观察凋亡细胞的形态学变化；运用流式细胞技术检测细胞凋亡率和细胞内活性氧水平；通过比色法测定细胞内caspase-9及caspase-3的相对活性。结果 大黄素以时间-剂量的方式抑制胆管癌QBC939细胞的增殖；在荧光显微镜下，凋亡的细胞呈现亮色，或核呈分叶、碎片状，细胞皱缩；QBC939细胞在30 µmol/L和50 µmol/L浓度的大黄素作用下，24 h的凋亡率分别为38.9%±9.07%及67.09%±4.08%（P<0.05），30 min后细胞内活性氧的水平分别为对照组的1.65±0.08及2.28±0.04倍（P<0.05）；大黄素可激活caspase-9及caspase-3，导致其活性升高（P<0.05）。结论 大黄素能通过诱导凋亡来抑制胆管癌QBC939细胞的增殖，其机制与细胞的活性氧水平升高及caspase-9及caspase-3活化有关。

关键词: 大黄素, 细胞凋亡, 活性氧, 半胱氨酸天冬氨酸蛋白酶

Abstract: Objective  To investigate the effect and mechanism of emodin inducing the apoptosis of human bile duct carcinoma cell QBC939 in vitro. Methods  The inhibition of cell proliferation was detected by MTT. The morphological changes of apoptotic cells were observed under fluorescence microscopy. The apoptosis rates and intracellular reactive oxygen species levels were detected by flow cytometry. The intracellular relative activities of caspase-9 and caspase-3 were tested through colorimetric method. Results  Emodin inhibited cell proliferation of human bile duct carcinoma cell QBC939 in time-dose dependent fashion. Apoptotic cells displayed bright, nuclear presented divided leaves, debris and cell shrinkage under fluorescence microscopy. Treated with 30 µmol/L and 50 µmol/L emodin, the apoptosis rates of 24 hours were respectively 38.9%±9.07% and 67.09%±4.08% (P<0.05). The intracellular reactive oxygen species levels after 30 minutes treatment were 1.65±0.08 and 2.28±0.04 folds of the control group (P<0.05). Emodin could activate caspase-9 and caspase-3, leading to elevations of their activities (P<0.05). Conclusions  Emodin inhibites cell proliferation of human bile duct carcinoma cell QBC939 through inducing apoptosis. The mechanism is associated with the elevated levels of reactive oxygen species and the activation of caspase-9 cells and caspase-3.

Key words: Emodin, Apoptosis, Reactive oxygen species, Caspases