国际肿瘤学杂志

国际肿瘤学杂志 ›› 2019, Vol. 46 ›› Issue (5): 267-271.doi: 10.3760/cma.j.issn.1673-422X.2019.05.003

• 论著 • 上一篇    下一篇

原发性胆囊癌患者血清miR-335和miR-155水平及其临床意义

吴唐1，李应明2，黄聪斐1，李小佩1   

  1. 1海南省第二人民医院检验科，五指山市572200； 2海口市人民医院检验科571700
  • 收稿日期:2019-01-10 出版日期:2019-05-08 发布日期:2019-06-14
  • 通讯作者: 李应明 E-mail:sjcszx999@163.com
  • 基金资助:

    海南省医学科研基金（17A601128）

Serum levels and significances of miR-335 and miR-155 in primary gallbladder cancer

Wu Tang1, Li Yingming2, Huang Congfei1, Li Xiaopei1   

  1. 1Department of Clinical Laboratory, Second People′s Hospital of Hainan Province, Wuzhishan 572200, China; 2Department of Clinical Laboratory, Haikou People′s Hospital, Haikou 571700, China
  • Received:2019-01-10 Online:2019-05-08 Published:2019-06-14
  • Contact: Li Yingming E-mail:sjcszx999@163.com
  • Supported by:

    Hainan Medical Research Fund of China (17A601128)

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摘要： 目的 探讨原发性胆囊癌（PCG）患者血清微小RNA-335（miR-335）和微小RNA-155（miR-155）水平及其临床意义。方法 选取2016年1月至2018年10月海南省第二人民医院收治的96例PCG患者（PCG组）和50例体检健康者（对照组），采用实时定量PCR（RTPCR）检测各组血清miR335和miR155水平，分析血清miR-335和miR-155水平与PCG患者临床病理特征的关系。应用ROC曲线分析miR-335和miR-155对PCG的诊断价值。结果 PCG组血清miR-335水平明显低于对照组（1.50±0.42∶3.65±1.18，t=10.319，P＜0.001），PCG组血清miR-155水平明显高于对照组（3.18±0.61∶0.74±0.12，t=13.627，P＜0.001）。在PCG患者中血清miR-335和miR-155水平均与TNM分期（t=4.863，P=0.024；t=5.117，P=0.008）及淋巴结转移（t=5.725，P＜0.001；t=6.802，P＜0.001）相关。ROC曲线分析显示，miR335和miR155诊断PCG的临界值分别为1.18、2.35，两项联合诊断PCG的曲线下面积最大，为0.920，95%CI为0.863～0.977，其敏感性和特异性为93.8%和85.7%。结论 血清低水平miR-335和高水平miR-155与PCG较高的TNM分期及淋巴结转移相关，两项联合检测有助于提高PCG诊断率。

关键词: 胆囊肿瘤, 微RNAs

Abstract: Objective To investigate the  serum levels and clinical significances of microRNA-335 (miR-335) and microRNA-155 (miR-155) in patients with primary gallbladder cancer (PCG). Methods A total of 96 PCG patients (PCG group) and 50 healthy controls (control group) admitted to the Second People′s Hospital of Hainan Province from January 2016 to October 2018 were selected. Real-time quantitative PCR (RT-PCR) was used to detect the serum levels of miR-335 and miR-155 in each group. The relationships between miR335 and miR-155 levels and clinical pathological characteristics of PCG patients were analyzed. The diagnostic value of miR-335 and miR-155 in PCG was analyzed by ROC curve. Results The serum level of miR-335 in PCG group was significantly lower than that in the control group (1.50±0.42 vs. 3.65±1.18, t=10.319, P＜0.001). The serum level of miR-155 in PCG group was significantly higher than that in the control group (3.18±0.61 vs. 0.74±0.12, t=13.627, P＜0.001). The serum levels of miR-335 and miR-155 in PCG patients were correlated with TNM stage (t=4.863, P=0.024; t=5.117, P=0.008) and lymph node metastasis (t=5.725, P＜0.001; t=6.802, P＜0.001). ROC curve analysis showed that the critical values of serum miR-335 and miR-155 for diagnosing PCG were 1.18 and 2.35, respectively. The area under the curve of the two combined diagnosis of PCG (0.920, 95%CI: 0.863-0.977) was the largest, with sensitivity and specificity of 93.8% and 85.7%. Conclusion The low serum level of miR335 and high level of miR155 are associated with the higher TNM stage and lymph node metastasis of PCG, and the combined detection of the two is helpful to improve the diagnostic rate of PCG.

Key words: Gallbladder neoplasms, MicroRNAs