Y盒结合蛋白1表达上调与宫颈癌上皮间质转化之间的相关性研究

doi:10.3760/cma.j.issn.1673422X.2015.05.004

摘要/Abstract

摘要： 目的探讨Y盒结合蛋白1(YB1)在宫颈癌中表达的临床病理意义及与上皮间质转化的相关性。方法选取202例标本，包括50例正常宫颈组织，100例宫颈上皮内瘤变(CIN)及52例鳞状细胞癌(SCC)，进行YB1和上皮型钙黏蛋白（Ecadherin）的免疫组织化学分析。结果 Ecadherin在正常宫颈组及膜表达率为100%，CINⅡ~Ⅲ组为64%，SCC组仅为3.85%，正常宫颈/CINⅠ组比CINⅡ~Ⅲ、正常/CINⅠ比SCC、CINⅡ~Ⅲ比SCC分别为χ2=40.909；χ2=119.088；χ2=25.274；均P<0.05。Ecadherin阴性及畸形表达率在转移SCC组和非转移SCC组中分别为100.0%（20/20）和68.75%（22/32），连续性校正性χ2=5.857，P=0.016。YB1在SCC病例胞质中表达为94.23%（49/52），CINⅡ~Ⅲ组表达率为10.00%（5/50），正常宫颈组和CINⅠ组表达率为0（0/100），正常宫颈/CINⅠ组比CINⅡ~Ⅲ、正常/CINⅠ比SCC、CINⅡ~Ⅲ比SCC分别为χ2=72.591；χ2=139.059；χ2=5.857；均P<0.05。在宫颈癌的进展过程中，YB1在早期SCC胞质表达率为60.71%（17/28），晚期SCC为91.67%（22/24），两组相比差异有统计学意义（χ2=6.603，P=0.01）。转移组和非转移组中分别为100.00%（20/20）和59.38%（19/32），两组相比差异有统计学意义（χ2=10.833，P=0.001）。结论 YB1过表达和宫颈上皮的恶性转化、分期进展及转移能力相关，YB1过表达也与Ecadherin低表达相关，可能是预测CIN癌变和宫颈癌侵袭转移的标志物。

Abstract: ObjectiveTo investigate the clinicopathologic significance of Ybox binding protein1 (YB1) expression in cervical cancer and its correlation with epithelialmesenchymal transition. MethodsA series of 202 samples, including 50 cases of normal cervical tissues, 100 cases of cervical intraepithelial neoplasia (CIN) and 52 cases of squamous cell carcinoma (SCC), were examined YB1 and Ecadherin by immunohistochemical staining. ResultsThe Ecadherin cell membrane immunoreactivity for normal/CINⅠ, CINⅡⅢ, and SCC tissues were 100%, 64% and 3.85%, respectively （χ2=40.909; χ2=119.088; χ2=25.274; P<0.05）. The negative and aberrant expression of Ecadherin was higher in metastatic SCC (100.0%, 20/20）than that in nonmetastatic SCC (68.75%, 22/32）(χ2=5.857, P=0.016). 94.23% （49/52） cases of SCC exhibited strong YB1 cytoplasmic immunoreactivity. The positive rates in normal/CINⅠand CINⅡⅢ were 0 (0/100）and 10.00% （5/50）（χ2=72.591； χ2=139.059； χ2=5.857; P<0.05）. The cytoplasmic expression of YB1 was higher in metastatic SCC (100.0%, 20/20）than that in nonmetastatic SCC (59.38%, 19/32）（χ2=10.833， P=0.001）. The rates were 60.71% （17/28） and 91.67% （22/24） in early stage SCC and late stage SCC （χ2=6.603, P=0.01）. ConclusionYB1 overexpression is associated with the malignant transformation of cervical epithelium, stage progression and metastasis of cervical cancer. The upregulation of YB1 is also associated with the downregulation of Ecadherin, and it may predict the malignant transformation of CIN and distal metastasis of cervical cancer.

李旻, 关弘, 胡新荣, 王鹰, 魏茜, 杨青凤. Y盒结合蛋白1表达上调与宫颈癌上皮间质转化之间的相关性研究[J]. 国际肿瘤学杂志, 2015, 42(5): 331-335.

LI Min, GUAN Hong, HU Xin-Rong, WANG Ying, WEI Qian, YANG Qing-Feng. Correlation of YB1 upregulation and epithelialmesenchymal transition during tumorigenesis and progression of cervical carcinoma[J]. Journal of International Oncology, 2015, 42(5): 331-335.

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