国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (10): 583-587.doi: 10.3760/cma.j.issn.1673-422X.2018.10.002

• 论著 • 上一篇    下一篇

洛铂对宫颈癌CaSki细胞增殖侵袭的影响

李翡,贺丰杰,朱虹丽,李楠,肖新春,李小宁,陈梅,郑伟,杨丽丽   

  1. 712000咸阳，陕西中医药大学妇产科学教研室（李翡、贺丰杰、朱虹丽、李楠、肖新春、李小宁、陈梅、杨丽丽）；西安市职工大学办公室（郑伟）
  • 收稿日期:2017-12-15 出版日期:2018-10-08 发布日期:2018-12-21
  • 通讯作者: 郑伟 E-mail:13595024@qq.com
  • 基金资助:

    咸阳市科技计划（2014K04-19）；陕西中医药大学附属医院科研课题（2014-09）

Effects of lobaplatin on proliferation and invasion of cervical cancer CaSki cells

Li Fei, He Fengjie, Zhu Hongli, Li Nan, Xiao Xinchun, Li Xiaoning, Chen Mei, Zheng Wei, Yang Lili   

  1. Department of Obstetrics and Gynecology, Shaanxi University of Traditional Chinese Medicine, Xianyang 712000, China
  • Received:2017-12-15 Online:2018-10-08 Published:2018-12-21
  • Contact: Zheng Wei E-mail:13595024@qq.com
  • Supported by:

    Science and Technology Program of Xianyang City of China (2014K04-19); Research Project of Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine (2014-09)

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摘要： 目的  探讨洛铂对宫颈癌CaSki细胞增殖侵袭的影响。方法  选取人宫颈癌CaSki细胞株，采用随机数字表法将其随机分为空白对照组、2、6、12 μg/ml洛铂组，采用四甲基偶氮唑蓝（MTT）法检测细胞增殖情况，采用倒置显微镜观察细胞形态学变化，采用Transwell侵袭实验检测细胞侵袭能力，采用Western blotting检测细胞外调节蛋白激酶（ERK）和磷酸化细胞外调节蛋白激酶（p-ERK）蛋白表达。结果  空白对照组、2、6、12 μg/ml洛铂组培养24 h的吸光度（A）值分别为0.513±0.023、0.428±0.014、0.380±0.012、0.300±0.013；培养48 h的A值分别为0.831±0.024、0.558±0.019、0.415±0.015、0.088±0.009；培养72 h的A值分别为1.153±0.022、0.572±0.023、0.201±0.017、0.052±0.014，差异均具有统计学意义（F=12.922，P＜0.001；F=10.192，P＜0.001；F=11.192，P＜0.001），进一步两两比较发现，差异均具有统计学意义（均P＜0.05）。倒置显微镜下可观察到洛铂组细胞皱缩变圆，体积缩小，形态不规则，细胞数量减少，间隙增大，且随洛铂浓度及作用时间的增加，这种变化越明显。空白对照组、2、6、12 μg/ml洛铂组穿膜细胞数分别为87.27±9.38、71.02±8.92、53.20±10.02、21.02±7.37，差异有统计学意义（F=87.291，P＜0.001），进一步两两比较发现，差异均具有统计学意义（均P＜0.05）。空白对照组、2、6、12 μg/ml洛铂组CaSki细胞ERK蛋白A值（0.955±0.021、0.953±0.023、0.950±0.020、0.951±0.022）比较差异无统计学意义（F=2.033，P=0.783）；空白对照组、2、6、12 μg/ml洛铂组CaSki细胞p-ERK蛋白A值分别为0.941±0.015、0.831±0.020、0.620±0.019、0.493±0.017，组间比较差异有统计学意义（F=11.921，P＜0.001），进一步两两比较发现，差异均具有统计学意义（均P＜0.05）。结论  洛铂能显著抑制宫颈癌CaSki细胞增殖侵袭，可能与其抑制pERK蛋白表达有关。

关键词: 宫颈肿瘤, 细胞增殖, 肿瘤侵润, 洛铂, CaSki细胞

Abstract: Objective  To investigate the effects of lobaplatin on proliferation and invasion of cervical cancer CaSki cells. Methods  Human cervical cancer CaSki cells were randomly divided into blank control group, 2, 6 and 12 μg/ml lobaplatin groups by random number table method. The proliferations of the cells were detected by methyl thiazolyl tetrazolium (MTT). The morphological changes of the cells were observed by inverted microscope. The invasive abilities of the cells were detected by Transwell invasion test. The protein expressions of extracellular signalregulated kinase (ERK) and phosphoextracellular signalregulated kinase (p-ERK) were detected by Western blotting. ResultsThe absorbance (A) values of blank group, 2, 6, 12 μg/ml lobaplatin groups cultured for 24 h were 0.513±0.023, 0.428±0.014, 0.380±0.012 and 0.300±0.013 respectively, those of the cells cultured for 48 h were 0.831±0.024, 0.558±0.019, 0.415±0.015 and 0.088±0.009 respectively, and those of the cells cultured for 72 h were 1.153±0.022, 0.572±0.023, 0.201±0.017 and 0.052±0.014 respectively. The differences were statistically significant (F=12.922, P＜0.001; F=10.192, P＜0.001; F=11.192, P＜0.001), and the differences between each two groups were statistically significant (all P<0.05). Under inverted microscope, the cells of the platinum groups were shrunken and round, the volume and quantity were reduced, the morphology was irregular, the gap was increased, and the changes were more obvious with the increase of the concentration and the culture time. The numbers of penetrating cells of the blank group, 2, 6, 12 μg/ml lobaplatin groups were 87.27±9.38, 71.02±8.92, 53.20±10.02 and 21.02±7.37 respectively. The difference was statistically significant (F=87.291, P＜0.001), and the differences between each two groups were statistically significant (all P<0.05). The A values of ERK protein in the blank group, 2, 6, 12 μg/ml lobaplatin groups （0.955±0.021、0.953±0.023、0.950±0.020、0.951±0.022） showed no significant difference (F=2.033, P=0.783), but the A values of p-ERK protein in the four groups were 0.941±0.015, 0.831±0.020, 0.620±0.019 and 0.493±0.017 respectively, which showed significant difference (F=11.921, P＜0.001), and the differences between each two groups were statistically significant (all P<0.05). Conclusion Lobaplatin can inhibit the proliferation and invasion of cervical cancer CaSki cells, which may be related to the inhibition of the expression of p-ERK protein.

Key words: Uterine cervical neoplasms, Cell proliferation, Neoplasm invasiveness, Lobaplatin, CaSki cells