国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (1): 1-4.doi: 10.3760/cma.j.issn.1673-422X.2015.01.001

• 论著 •    下一篇

老年喉癌患者外周血Treg/Th17细胞表达失衡的检测及其意义

林丹琪,阙镇如,蔡继一,黄钦辉,谢怡   

  1. 解放军第180医院耳鼻咽喉科

  • 收稿日期:2014-08-18 修回日期:2014-10-14 出版日期:2015-01-08 发布日期:2015-01-07
  • 通讯作者: 阙镇如 E-mail:adanlover@163.com
  • 基金资助:

    南京军区医药卫生科研基金（12MA071）

Imbalance of Treg/Th17 cells in peripheral blood of elderly patients with laryngeal cancer and its significance

Lin Danqi, Que Zhenru, Cai Jiyi, Huang Qinhui, Xie Yi   

  1. Department of Otorhinolaryngology, the 180th Hospital of People’s Liberation Army, Quanzhou 362000, China
  • Received:2014-08-18 Revised:2014-10-14 Online:2015-01-08 Published:2015-01-07
  • Contact: Que Zhenru E-mail:adanlover@163.com

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摘要： 目的 探讨老年喉癌患者外周血中CD4+CD25+叉状头转录因子p3（Foxp3）+ 调节性T细胞（Treg）和CD4+白细胞介素（IL）-17+辅助性T细胞17（Th17）的表达特点及其与老年喉癌患者病程进展的关系。方法选取2013年1月至2014年6月在解放军第180医院耳鼻喉科确诊为喉癌的老年患者60例，其中伴颈部淋巴结转移28例，无颈部淋巴结转移32例，另取同期无喉部疾病的健康人30例作为对照。分别抽取老年喉癌患者及正常对照者外周血，以流式细胞术检测各组人群外周血中Treg和Th17细胞表达水平，实时定量聚合酶链反应检测外周血单核细胞（PBMC）中Foxp3、维甲酸相关核孤儿受体（RORγt）基因表达变化。结果与对照组相比，无淋巴结转移老年喉癌患者Treg细胞百分比（t=9.817，P=0.018）、Th17细胞百分比（t=12.120，P=0.009）、Foxp3（t=6.090，P=0.023）和RORγt（t=7.130，P=0.028）表达水平均有所升高。伴淋巴结转移老年喉癌患者与无淋巴结转移老年喉癌患者相比，Treg细胞百分比（t=9.070，P=0.014）、Th17细胞百分比（t=12.140，P=0.009）和Foxp3（t=10.130，P=0.009）、RORγt（t=13.070，P=0.008）表达水平均显著升高，差异均有统计学意义。而伴淋巴结转移老年喉癌患者Treg/Th17和Foxp3/RORγt比显著低于无淋巴结转移老年喉癌患者（2.401±0.614 vs 3.763±0.959; 0.401±0.075 vs 0.563±0.091），差异有统计学意义（t=9.070，P=0.014; t=11.140，P=0.007）。结论老年喉癌患者中Treg细胞和Th17细胞表达失衡程度与老年性喉癌患者病情的进展呈正相关。因此，监测及纠正老年喉癌患者Treg和Th17细胞水平对于本疾病的辅助诊断、治疗及预后有积极作用。

关键词: 喉肿瘤, Treg, Th17, Foxp3, RORγt

Abstract: ObjectiveTo investigate the relationship of the expression characteristics of CD4+CD25+Foxp3+ Treg cells and CD4+IL17+ Th17 cells and the progress of the elderly patients with laryngeal cancer. MethodsSixty elderly patients diagnosed as laryngeal cancer in our hospital from January 2013 to June 2014 were chosen. Among them, 28 cases had lymph node metastasis and 32 without. Thirty cases without laryngeal diseases were chosen as control. The peripheral blood of elderly patients with laryngeal cancer and normal controls was extracted. The expression levels of Treg and Th17 cells in all groups were detected with flow cytometry, and the expression changes of Foxp3, RORγt in peripheral blood mononuclear cell (PBMC) were detected with real time quantitative PCR. ResultsCompared with the control group, CD4+CD25+Foxp3+ Treg and CD4+IL17+ Th17 cell percentage, Treg and Th17 cells specific transcription factor Foxp3, RORγt expression levels in elderly laryngeal cancer patients without lymph node metastasis were significantly higher (t=9.817, P=0.018; t=12.120, P=0.009; t=6.090, P=0.023; t=7.130, P=0.028). Compared with elderly laryngeal cancer patients without lymph node metastasis, Treg and Th17 cell percentage and Foxp3, RORγt expression levels were significantly increased (t=9.070, P=0.014; t=12.140, P=0.009; t=10.130, P=0.009; t=13.070, P=0.008), and the ratios of Th17/Treg and Foxp3/RORγt were significantly lower (2.401±0.614 vs 3.763±0.959; 0.401±0.075 vs 0.563±0.091;t=9.070，P=0.014; t=11.140, P=0.007) in elderly laryngeal cancer patients with lymph node metastasis. ConclusionIn elderly patients with laryngeal cancer, Treg cells and Th17 cells imbalance is positively correlated to disease progression. Therefore, monitoring and correcting the expression levels of Treg and Th17 cells may be important for the diagnosis, treatment and prognosis of the elderly patients with laryngeal cancer.

Key words: Laryngeal neoplasms, Treg, Th17, Foxp3, RORγt