国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (8): 708-711.doi: 10.3760/cma.j.issn.1673-422X.2014.09.019

• 论著 • 上一篇    下一篇

微小RNA表达比在骨肉瘤中的预测作用

任慧文,杨澄,苏宏伟,李宏伟   

  1. 130021 长春，吉林大学中日联谊医院骨外科
  • 出版日期:2014-09-25 发布日期:2014-09-05
  • 通讯作者: 李宏伟 E-mail:lihw@163.com

Predictive effect of microRNA ratio in osteosarcoma

Ren Huiwen, Yang Cheng, Su Hongwei, Li Hongwei   

  1. Department of Orthopedic Surgery, ChinaJapan Union Hospital, Jilin University, Changchun 130021, China
  • Online:2014-09-25 Published:2014-09-05
  • Contact: Li Hongwei E-mail:lihw@163.com

PDF

1525

被引次数

2

摘要： 目的探讨微小RNA（miRNA）表达比在骨肉瘤中的生物标志物作用。方法选取经病理证实为骨肉瘤患者的血样22例，年龄相近的30例健康者血样作为对照组。运用高通量芯片的方法对差异表达的miRNA进行筛选，对分析结果进行qRTPCR验证；用受试者工作特征曲线分析miRNA表达比，找出最具预测潜能的组合型标志物。结果与正常血样相比，基因芯片检测出258个差异表达的miRNA（F=5.564，P＜0.05），选取其中4个miRNA在22例骨肉瘤患者血样和正常对照组血样中进行验证。其中miR181b、miR199b5p、miR451在骨肉瘤血样中的表达较正常血样明显增高(F=6.283，P＜0.05)，而miR124在骨肉瘤中则是低表达(F=7.201，P＜0.05)；miR199b5p/miR124的表达比表现出很高的敏感性（96%）和特异性（97%）。结论miRNA在骨肉瘤中的异常表达使其具备一定的预测作用，而miR199b5p/miR124表达比或许可以成为预测骨肉瘤的生物标志物。

关键词: 微RNAs, 骨肉瘤, 生物学标记

Abstract: ObjectiveTo explore the roles of biomarkers of the ratios of microRNA (miRNA) in osteosarcoma. MethodsThe blood samples from 20 patients with osteosarcoma and 30 healthy people with similar age were choosed. The miRNA expression profiles were filtered by gene chips of high flux. The results were verified by qRTPCR. The miRNA expression ratio was analysed by receiver operating characteristic (ROC) curve aiming to find the most predictive potential combinationtype markers. ResultsTwo hundred and fiftyeight miRNAs were found to be significantly deregulated in blood samples from 30 patients with osteosarcoma as compared with their matching normal blood samples (F=5.564, P＜0.05). Four miRNAs were choosed from blood samples of 22 patients with osteosarcoma and normal blood samples. MiR181b, miR199b5p and miR451 were significantly higher than that in normal blood sample (F=6.283，P＜0.05), while miR124 showed low expression (F=7.201，P＜0.05). Furthermore, the ratio of miR199b5p/miR124 showed a high sensitivity (96%) and specificity (97%). ConclusionThe abnormal expression of miRNA in osteosarcoma makes it have a predictive effect. The ratio of miR199b5p/miR124 could be served as combinationtype biomarkers in the diagnosis of osteosarcoma.

Key words: MicroRNAs, Osteosarcoma, Biological markers