Abstract: Objective  To confirm the effect of miR-93 inhibitor in glioma cell growth.  Methods  Malignant glioma cells were transfected with miR-93 inhibitor by lipofectamin to downregulate their overexpression of miR-93. Real time-PCR was taken to measure miR-93 expression after transfection. The cell cycle kinetics and cell growth rate were detected by flowcytometry and MTT assay, the cell proliferative ability was evaluated by soft agar assay, and the invasive ability was detected by transwell assay.  Results  The high-level expression of miR-93 was downregulated effectively in glioma cells after transfecting the miR-93 inhibitor. Meanwhile, the cell cycle progress was delayed, S phase cells were reduced, the speed of growth was slowed, cloning formation ability was receded, the number of cells through the matrigel was reduced, and invasive ability was significantly repressed. Conclusion  Downregulation of miR-93 expression could inhibit the proliferative ability and invasive ability of glioma cells.