Abstract: Objective  To test the expression of epidermal growth factor-like domain 7 (EGFL7), microvessel density (MVD) and foeal adhesion kinase pY397 (FAKpY397) in human glioma tissues, and to evaluate their relationship. Methods  The expression of EGFL7 and FAKpY397 in 56 cases of human glioma and 8 cases of normal brain tissues were detected by immunohistochemistry test, and MVD was detected by CD34 staining. Results  There was a significant difference of the positive rates of EGFL7 between normal brain tissue and gliomas (P<0.01). With the increased pathological grade, the expression level of EGFL7 increased (P<0.01). There was a significant difference of the positive rates of FAKpY397 between normal brain tissue and gliomas (P<0.05). With the increased pathological grade, the expression level of FAKpY397 increased (P<0.01). There was a significant difference on MVD between normal brain tissue and gliomas (P<0.01). Higher level of MVD was found in gliomas with higher grade (P<0.01). There was a positive correlation between EGFL7 and FAKpY397 expressions in gliomas (P<0.01). There was a significant difference on MVD between positive and negative expression of EGFL7 (P<0.01). Conclusion  The expression of EGFL7 of human gliomas has a favorable positive correlation with the degree of malignancy, MVD and FAKpY397. It is indicated that EGFL7 not only palys an important regulative role in glioma neovascularization, also it may participate directly in glioma occurrence and invasion.