国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (5): 375-379.doi: 10.3760/cma.j.issn.1673-422X.2014.05.017

• 论著 • 上一篇    下一篇

半乳糖凝集素3对食管癌Eca109细胞增殖和转移的影响

梁宁,谢健,乔丽丽,张建东   

  1. 261061潍坊医学院研究生院（梁宁）；山东大学医学院研究生院（谢健、乔丽丽）；山东大学附属千佛山医院肿瘤放疗科（张建东）
  • 收稿日期:2013-12-05 修回日期:2014-02-25 出版日期:2014-05-08 发布日期:2014-04-28
  • 通讯作者: 张建东 E-mail:zhangjd165@sina.com

Effect of galectin3 on proliferation and migration of esophageal cancer Eca109 cells

Liang Ning, Xie Jian, Qiao Lili, Zhang Jiandong   

  1. Graduate School, Weifang Medical College, Weifang 261061, China
  • Received:2013-12-05 Revised:2014-02-25 Online:2014-05-08 Published:2014-04-28
  • Contact: Zhang Jiandong E-mail:zhangjd165@sina.com

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5

摘要： 目的 探讨半乳糖凝集素3（Gal3）对食管癌Eca109细胞增殖和转移的影响。方法 构建合成Gal3过表达慢病毒质粒转染食管癌Eca109细胞，应用倒置显微镜观察Gal3的过表达质粒转染食管癌细胞后荧光表达；CCK8法检测转染前后细胞增殖能力的变化；用流式细胞术检测转染组细胞和未转染组细胞凋亡率。Transwell方法检测转染前后细胞迁移能力变化；用Western印迹检测Gal3转染前后在食管癌中表达水平变化。结果 Western印迹结果显示Gal3在食管癌细胞中表达，在Eca109/Gal3组中表达水平明显升高（t=14.33，P=0.013；t=10.28, P=0.037）。CCK8法检测发现转染后细胞增殖能力明显升高(t=-17.277，P＜0.05；t=-13.4， P＜0.05)，流式细胞仪以AnnexinV/7AAD双标法显示Eca109/Gal3组凋亡率明显下降（t=3.053，P＜0.05；t=5.446, P＜0.05）。Transwell实验结果，Eca109/Gal3组细胞转移率高于未转染组（t=3.465，P＜0.05； t=3.252, P＜0.05）。结论 Gal3在食管癌细胞中有表达，并且其过表达能显著增强食管癌细胞的增殖、侵袭及迁移能力，明显抑制细胞的凋亡，深入研究Gal3可能为食管癌的治疗提供一种新的靶点。

关键词: 食管肿瘤, 半乳糖凝集素3, RNA过表达, 生物学功能

Abstract: Objective To investigate galectin3 on proliferation and migration of esophageal cancer Eca109 cells. Methods A lentiviral vector for overexpression of RNA targeting galectin3 was designed to transfect Eca109 cancer cells following plasmidmediated transfection manual (Eca109/Gal3 cells). Inverted fluorescence microscope was used to observe the expression of EGFP. The proliferation of Eca109 cells was measured by cell counting Kit8 assay. Eca109 cells apoptosis was determined by AnnexinV/7AAD doublestaining. The migration capacity of Eca109 cells was determined in transwell assays. Western blot analysis was used to measure the expression of galectin3 protein. ResultsGalectin3 expression was detected in Eca109 cells, with the Galectin3 expression in Eca109/Gal3 cells much more than nontransfected cells (t=14.33， P＜0.05； t=10.28, P=0.037). Compared with nontransfected Eca109 cells, proliferation increased significantly in Eca109/Gal3 cells (t=-17.277， P＜0.05； t=-13.4， P＜0.05). Galectin3 evidently decreased in Eca109 cell apoptosis (t=3.053， P＜0.05; t=5.446, P＜0.05). Transwell migration assay showed that a greater number of Eca109/Gal3 cells crossed the artificial basement membrane compared with nontransfected Eca109 cells and negative control Eca109 cells (t=3.465, P＜0.05; t=3.252, P＜0.05). Conclusion Galectin3 expression is detected in transfected esophageal cancer Eca109 cells, whose overexpression can result in enhanced proliferation, migration, invasion as well as reduced apoptosis. These data indicate that indepth research of galectin3 may prove to be a potential molecular target for the treatment of esophageal cancer.

Key words: Esophageal neoplasms, Galectin 3, RNA over-expression, Biological function