国际肿瘤学杂志

国际肿瘤学杂志 ›› 2012, Vol. 39 ›› Issue (12): 950-952.

• 论著 • 上一篇    下一篇

食管鳞癌组织中诱导型一氧化氮合酶和血管内皮生长因子的表达及意义

张雷   

  1. 271000山东，泰安市中心医院胸外科
  • 出版日期:2012-12-08 发布日期:2012-11-15

Expression and significance of iNOS and VEGF in human esophageal squamous cell carcinoma

ZHANG  Lei   

  1. Department of Thoracic Surgery, Tai’an City Central Hospital, Taian,271000, China
  • Online:2012-12-08 Published:2012-11-15

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1584

被引次数

8

摘要： 目的 研究食管鳞癌组织中诱导型一氧化氮合酶（iNOS）与血管内皮生长因子(VEGF)的表达及其临床意义，探讨iNOS与VEGF的相关性。方法  用免疫组化SABC法检测52例食管鳞癌组织和20例癌旁正常食管组织中iNOS与VEGF的表达，并统计分析两者的相关性及其与食管癌临床病理学特征的关系。结果 iNOS与VEGF在食管鳞癌组织中的阳性率分别是63.46％(33/52)和67.31％(35/52)，在癌旁正常组织中的阳性率分别是10%（2/20）和20%（4/20），在食管鳞癌组织中的表达水平明显高于癌旁组织中的表达水平（P﹤0.01）。 iNOS与VEGF的表达与食管鳞癌的组织学分级（P﹤0.01）、临床TNM分期和淋巴结转移有关（P﹤0.05），与患者性别无关（P＞0.05），iNOS的表达与VEGF的表达呈正相关（ｒ＝0.748 2，P﹤0.01）。结论 iNOS与VEGF蛋白在食管鳞癌组织中均有较高的阳性表达，且表达有相关性，两者可能存在共同的激活机制，iNOS和VEGF蛋白表达与食管癌的恶性进程有关。

关键词: 食管肿瘤, 一氧化氮合酶, 血管内皮生长因子类

Abstract: Object  To investigate the expression of inducible nitric oxide synthase(iNOS)and vascular endothelial growth factor(VEGF) in human esophageal squamous cell carcinoma and their clinical significances,and to evaluate the correlation between the expression of iNOS and VEGF.   Methods  Immunohistochemical SABC methond was used to detect the expression of iNOS and VEGF in 52 cases of esophageal squamous cell carcinoma and 20 cases of paired para-carcinama. The correlation between the expression of iNOS and VEGF was analyzed statistically. And the correlation between the expression of iNOS and VEGF and the clinical pathological features of the patients was analyzed statistically.  Results  The positive expression rates of iNOS and VEGF in esophageal squamous cell carcinoma were 63.46％(33/52) and 67.31％(35/52),respectively. The positive expression rates of iNOS and VEGF in paired para-carcinama tissues were 10%（2/20）and 20%（4/20）respectively. The positive expression rates of iNOS and VEGF in esophageal squamous cell carcinoma were significantly higher than those in paired para-carcinama tissues（P﹤0.01）. There was significiat correlation  between the expression of  iNOS, VEGF and the pathological grade（P﹤0.01）,clinic TNM stage（P﹤0.05）, lymph noed metastasis（P﹤0.05），but there was no correlation between the expression of iNOS, VEGF and the patients sex（P＞0.05）. Expression of iNOS was positively correlated with that of VEGF（ｒ＝0.7482，P﹤0.01）.  Conclusion   iNOS and VEGF are highly expressed and correlated in esophageal carcinoma. It is speculated that a common activation mechanism may coexists between the two genes. The expression of iNOS and VEGF plays an important role in the malignant progression.

Key words: Esophageal neoplasms, Nitric oxide synthas, Vascular endothelial growth factors