国际肿瘤学杂志

国际肿瘤学杂志 ›› 2012, Vol. 39 ›› Issue (7): 551-554.

• 论著 • 上一篇    下一篇

霞草苷Ⅱ对前列腺癌细胞侵袭力的影响

王燕,王风玲, 牟艳玲,李军   

  1. 济南，山东省医学科学院药物研究所山东省罕少见病重点实验室
  • 出版日期:2012-08-06 发布日期:2012-08-06

Effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma cells

WANG  Yan, WANG  Feng-Ling, MOU  Yan-Ling, LI  Jun   

  1. Institute of Materia Medica Shandong Academy of Medical Sciences, Key  Laboratory  for Rare Disease of Shandong Province, Jinan 250062, China
  • Online:2012-08-06 Published:2012-08-06

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摘要： 目的观察霞草苷Ⅱ对前列腺癌DU145细胞侵袭力的影响，并探讨其可能的作用机制.方法前列腺癌DU145细胞经霞草苷Ⅱ处理后，用四甲基偶氮唑蓝法检测其生长与增殖的变化；细胞侵袭小室法观察其体外侵袭能力的变化；免疫细胞化学方法研究其基质金属蛋白酶-2（MMP-2）、CXC 趋化因子受体4（CXCR4）的表达变化.结果经霞草苷Ⅱ处理后，DU145细胞的增殖受抑制；体外侵袭实验结果显示，对照组每100倍视野穿过的细胞数平均为180.3个±14.6个，而0.25、0.5、1 μg/ml 霞草苷Ⅱ作用24 h后，每100倍视野穿过的细胞数分别为100.4个±1.5个、80.2个±2.7个和60.1个±5.3个，比对照组明显减少，（P＜0.05）；免疫细胞化学显示，经霞草苷Ⅱ处理后，MMP-2表达下调，CXCR4表达下调.结论霞草苷Ⅱ抑制DU145细胞侵袭力的作用可能与其下调MMP-2的表达有关；霞草苷Ⅱ可能通过下调DU145细胞CXCR4的表达从而抑制癌细胞向骨转移。

关键词: 前列腺肿瘤, 基质金属蛋白酶类, 受体, CXCR4, 霞草苷Ⅱ

Abstract: ObjectiveTo observe the effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma DU-145 cells, and to investigate the potential mechanism. MethodsAfter treatment of DU-145 cells with oldhamianoside Ⅱ, MTT assay was used to test the effect of oldhamianoside Ⅱ on the cell proliferation.The invasive ability was assessed with a transwell cell culture chamber. Immunocytochemistry stain was used to investigate the expression of matrix metalloproteinase (MMP-2) and CXCchemokine receptor 4 (CXCR4). ResultsThe proliferation of DU-145 cells was inhibited after treatment with oldhamianoside Ⅱ. In vitro invasion assay indicated that the cells moved through the membrane were (180.3±14.6)／field in the control group, while decreased to (100.4±1.5), (80.2±2.7) and (60.1±5.3)／field in 0.25, 0.5 and 1 μg/mL oldhamianoside Ⅱ treated DU145 cells respectively. Comparing to control group, the cells of oldhamianoside Ⅱ groups moved through the membrane were decreased remarkably (P＜0.05). Immunocytochemistry showed that the expression of MMP-2 and CXCR4 were decreased of oldhamianoside Ⅱ groups. ConclusionsOldhamianoside Ⅱ can inhibit the invasiveness of DU-145 cells, which is related to the down regulation the expression of MMP-2 in DU-145 cells. Oldhamianoside Ⅱ can inhibit the prostate cancer cells metastasis to the bone, which is related to the down regulation the expression of CXCR4 in DU-145 cells.

Key words: Prostatic neoplasms, Matrix metalloproteinases, Receptors, CXCR4, Oldhamianoside Ⅱ