国际肿瘤学杂志

国际肿瘤学杂志 ›› 2017, Vol. 44 ›› Issue (4): 241-245.doi: 10.3760/cma.j.issn.1673422X.2017.04.001

• 论著 •    下一篇

白细胞介素-17对人喉癌细胞Hep-2的作用

冯舒,王郡甫,陈雪梅,栾俊文,苏庆红,栾萌,许晓群   

  1. 济南大学 山东省医学科学院医学与生命科学学院（冯舒）；山东省医学科学院基础医学研究所（王郡甫、栾俊文、苏庆红、栾萌、许晓群）；山东大学第二医院耳鼻咽喉头颈外科（陈雪梅）
  • 出版日期:2017-04-08 发布日期:2017-05-09
  • 通讯作者: 许晓群 E-mail:xuxiaoqunsd@163.com
  • 基金资助:

    山东省自然科学基金(ZR2013HL028,ZR2014HQ030,ZR2014HM085,ZR2015PC018)；山东省大型科学仪器设备升级改造技术研究专项（2013SJGZ09）；山东省医学科学院医药卫生科技创新工程；山东省中医药科技发展计划（2015-326） 

Effects of interleukin-17 on human laryngeal carcinoma Hep-2 cells

Feng Shu, Wang Junfu, Chen Xuemei, Luan Junwen, Su Qinghong, Luan Meng, Xu Xiaoqun   

  1. School of Medicine and Life Sciences, University of Ji′nanShandong Academy of Medical Sciences, Ji′nan 250062, China
  • Online:2017-04-08 Published:2017-05-09
  • Contact: Xu Xiaoqun E-mail:xuxiaoqunsd@163.com
  • Supported by:

    Natural Science Foundation of Shandong Province of China (ZR2013HL028, ZR2014HQ030, ZR2014HM085, ZR2015PC018); Special Research Foundation of Large Scientific Instruments on Upgrading and Technological Transformation of Shandong Province of China (2013SJGZ09); Innovation Project of Shandong Academy of Medical Sciences; Project of Medical and Health Technology Development Program of Shandong Province of China (2015-326)

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摘要： 目的 探讨白细胞介素-17(IL-17)对人喉癌细胞Hep-2增殖、凋亡和迁移的作用。方法 将IL-17瞬时转染Hep-2细胞，即转染IL-17组，同时设置空载体组(pEGFP-N1)和正常对照组。荧光显微镜观察转染情况，反转录聚合酶链反应（RT-PCR）和Western blotting分别检测转染前后IL-17 mRNA和蛋白的表达，四甲基偶氮唑蓝（MTT）法检测转染前后细胞增殖能力变化，流式细胞术检测转染前后细胞凋亡变化，细胞划痕修复实验和Transwell小室检测转染前后细胞迁移能力变化。结果 荧光显微镜下可以看到转染空载体pEGFP-N1和转染目的基因IL-17的Hep-2细胞出现绿色荧光。成功转染IL-17后Hep-2细胞在mRNA和蛋白水平均有IL-17的表达。与正常对照组相比，转染48 h后，转染IL-17组细胞的增殖能力明显减弱（0.34±0.03∶0.46±0.04，P=0.006）。转染IL-17组细胞凋亡率明显高于正常对照组（26.80%±0.80%∶2.90%±0.31%，P=0.000）。细胞划痕修复实验结果显示，转染IL17组细胞相对划痕宽度明显大于正常对照组（1.59±0.01∶1.36±0.01，P=0.000）。Transwell小室迁移实验结果显示，转染IL-17组细胞明显低于正常对照组细胞的迁移数量（26.33±2.08∶49.33±1.53，P=0.000）。结论 IL-17能够抑制人喉癌细胞Hep-2的增殖能力，降低其迁移能力，增强其凋亡。因此，IL-17可通过多种途径抑制喉癌的发生发展。

关键词: 白细胞介素17, 喉肿瘤, 细胞增殖, 细胞凋亡, 细胞运动

Abstract: Objective To investigate the effects of interleukin-17 (IL-17) on the cell proliferation, apoptosis and migration of human laryngeal carcinoma Hep-2 cells. Methods IL-17 was transiently transfected into Hep-2 cells, and at the same time empty vector group (pEGFP-N1) and normal control group were set up. The efficiency of transfection was evaluated by fluorescence microscope, and the mRNA and protein expressions of IL-17 were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The proliferation of cells was detected by methyl thiazolyl tetrazolium (MTT) method, and the apoptosis was detected by flow cytometry. The migration ability was detected by woundhealing assay and Transwell assay. Results  Hep-2 cells transfected with empty vector pEGFP-N1 and IL-17 showed green fluorescence under the fluorescence microscope. Hep-2 cells expressed IL-17 at both mRNA and protein levels after transfection with IL-17. Compared with the normal control group, the proliferation of IL-17 transfected Hep-2 cells was significantly inhibited after 48 h transfection (0.34±0.03 vs. 0.46±0.04, P=0.006). The apoptotic rate of IL-17 transfected cells was higher than that of normal control group (26.80%±0.80% vs. 2.90%±0.31%, P=0.000). According to the woundhealing assay, compared with the normal control group, the scratch width of IL-17 transfected cells was significantly greater (1.59±0.01 vs. 1.36±0.01, P=0.000). Transwell migration experiment showed that the migration of  IL-17 transfected cells was significantly lower than that of the normal control group (26.33±2.08 vs. 49.33±1.53, P=0.000). Conclusion IL-17 can inhibit the proliferation of human laryngeal carcinoma Hep-2 cells, reduce their migration ability and enhance their apoptosis ability. Therefore, IL-17 may inhibit the occurrence and development of laryngeal carcinoma through a variety of mechanisms.

Key words: Interleukin-17, Laryngeal neoplasms, Cell proliferation, Apoptosis, Cell movement