国际肿瘤学杂志

国际肿瘤学杂志 ›› 2011, Vol. 38 ›› Issue (11): 868-872.

• 论著 • 上一篇    下一篇

S100A4和uPA的表达及其与胰腺癌预后关系的研究

贾富鑫, 刘江伟, 张东, 等   

  1. 830000 兰州军区乌鲁木齐总医院肝胆外科（贾富鑫、刘江伟、张东、李鹏、冯玉玲），实验动物中心（李建英、卢开柏）

  • 出版日期:2011-11-08 发布日期:2011-11-03
  • 通讯作者: 刘江伟, E- mail: ljw273@sohu.com E-mail:ljw273@sohu.com
  • 基金资助:
    中国博士后科学基金第48批面上资助项目(20100481517)

Expressions of S100A4 and uPA and the correlation with pancreatic cancer prognosis

JIA  Fu-Xin, LIU  Jiang-Wei, ZHANG  Dong, et al    

  1. Department of Hepatobiliary Surgery,Urumuqi General Hospital of Lanzhou Military region,Urumuqi 830000,China
  • Online:2011-11-08 Published:2011-11-03

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1897

被引次数

7

摘要： 目的 探讨S100A4和尿激酶型纤溶酶原激活剂(uPA)在胰腺癌组织中的表达及其对患者预后的影响。方法 应用免疫组化二步法(PV法)检测63例手术切除的原发性胰腺癌组织中S100A4和uPA蛋白的表达和临床病理因素及预后的关系。结果 ①63例标本中S100A4和uPA阳性率分别为74.6%(47/63)、65.1%(44/63)。②S100A4、uPA蛋白过表达显著相关(P=0.000,r=0.567)。③S100A4表达与肿瘤的TNM分期(P=0.002)、淋巴结转移(P=0.002)及远处转移(P=0.007)显著相关；uPA表达与肿瘤的TNM分期(P=0.002)、淋巴结转移(P=0.001)及分化程度(P=0.003)显著相关。④Kaplan-Meier法描绘生存曲线分析表明，S100A4蛋白阴性组患者中位生存时间（21个月)明显大于阳性组(9个月),差异有统计学意义(P=0.000)；uPA蛋白阳性组患者中位生存时间(9个月)明显较阴性组(18个月)短, 差异有统计学差异(P=0.000)；S100A4阴性/uPA阴性组患者(13例)中位生存时间(23个月)明显高于其他组，差异有统计学意义(Log-rank检验,χ2=54.444,P=0.000)。⑤Cox比例风险模型(χ2=53.974,P=0.000)多因素分析表明，分化程度(P=0.004)、淋巴结转移(P=0.017）、S100A4阳性表达（P=0.000）及uPA阳性表达(P=0.001)为影响胰腺癌预后的独立因素。结论 胰腺癌组织中存在S100A4、uPA的高表达,且两者之间的表达强度具有等级相关性，其高表达提示患者预后不良。S100A4可能上调uPA表达以促进细胞外基质和基底膜的降解，最终促进肿瘤的侵袭和转移，不利于预后。联合检测二者表达可预测胰腺癌发生转移的危险性及患者预后情况。

Abstract: Objective To study the expressions of S100A4 and urokinase plasminogen activator(uPA)in pancreatic cancer cells and their correlation with patients prognosis. Methods The expressions of S100A4 and uPA were examined in 63 surgical specimens of primary pancreatic carcinoma with immunohistochemistry PV methods, and correlation of their expressions and prognosis of pancreatic cancer was analyzed．Results (1)Positive immunostaining for S100A4 and uPA was observed in 74.6%(47 cases) and 65.1%(44 cases) of 63 pancreatic cancer samples respectively. (2) The positive expressions of S100A4 and uPA were significantly correlated in pancreatic cancer(P=0.000，r=0.567).(3)The expression of S100A4 significantly correlated with TNM stages(P=0.002),lymph node metastasis(P=0.002)and distant metastasis(P=0.007).The expression of uPA had a significant correlation with TNM stages(P=0.002), lymph node metastasis(P=0.001）and differentiation（P=0.003). (4)Kaplan-Meier survival analysis showed that the median survival(21 months)of patients with S100A4(-)was significantly longer than the median survival(9 months)of the patients with S100A4(+)(P= 0.000);the median survival(9 months) of patients with uPA(+) was significantly shorter than the median survival (18 months) of the patients with uPA(-)(P=0.000);the median survival(23 months)of 13 patients with S100A4(-)/uPA(-)was significantly longer than the median survival of other cases(Log-rank test，χ2=54.444，P=0.000).(5)Cox regression model(χ2= 53.974, P=0.000）analysis suggested: the differentiation(P=0.004),lymph node metastasis(P=0.017)、S100A4(+) expression（P=0.000)and uPA(+)expression(P=0.001)were independent prognostic factors for pancreatic cancer. Conclusion S100A4 and uPA are highly expressed in pancreatic cancer cells, and S100A4 expression has positive correlation with uPA expression, which indicates that the overexpression of S100A4 and uPA maybe poor prognosis factors for pancreatic cancer patients. S100A4 maybe promote extracellular matrix and basement membrane degradation by up-regulation of uPA protein expression, and ultimately promote tumor invasion and metastasis, which is not favorable to prognosis.They may be potential indicators of metastasis and predictors for prognosis of pancreatic cancer.