Abstract: Proton magnetic resonance spectroscopy（HMRS） is the only way to study noninvasively the metabolic and biochemical signature of the human tissues and organs. It can also quantify the metabolites within the volume of interest. The metabolic information about the lesions provided by the HMRS can greatly improve the diagnostic, grading accuracy, treatment planning such as target delineation and posttreatment follow-up of the glioma.