国际肿瘤学杂志

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (3): 193-197.doi: 10.3760/cma.j.issn.1673-422X.2016.03.008

• 论著 • 上一篇    下一篇

宫颈癌及癌前病变中IL-10和IL-18的表达及临床意义

王云涛,曾贵林,黎斌,薛志红,曾守群   

  1. 611130 成都市第五人民医院肿瘤科
  • 出版日期:2016-03-08 发布日期:2016-02-03
  • 通讯作者: 曾守群，Email： wjqzsq@126.com E-mail:wjqzsq@126.com

Expressions and clinical significances of IL-10 and IL-18 levels in patients with cervical intraepithelial neoplasias and cervical carcinoma

Wang Yuntao, Zeng Guilin, Li Bin, Xue Zhihong, Zeng Shouqun   

  1. Department of Oncology, Fifth People′s Hospital of Chengdu, Chengdu 611130, China
  • Online:2016-03-08 Published:2016-02-03
  • Contact: Zeng Shouqun, Email: wjqzsq@126.com E-mail:wjqzsq@126.com

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摘要： 目的 探讨白细胞介素（IL）-10、IL-18在宫颈上皮内瘤变（CIN）及宫颈癌患者血清及组织中的表达及其临床意义。方法 收集108例（68例CIN、40例宫颈癌）患者的血液及临床组织标本，其中浸润性宫颈鳞状细胞癌（SCC）40例，CINⅠ期25例，CINⅡ～Ⅲ期43例，同时收集正常女性宫颈组织20例。通过酶联免疫吸附试验检测血清中IL-10和IL-18的表达，聚合酶链反应法检测宫颈组织中IL-10和IL-18的表达，第2代杂交捕获检测宫颈涂片中人乳头瘤病毒（HPV） DNA的表达情况，分析IL-10和IL-18表达与临床预后及HPV的关系。结果 IL-10和IL-18在正常对照组血清中的表达分别为（212.75±62.09）、（187.84±81.03）pg/ml，IL-10在CINⅠ期、CINⅡ～Ⅲ期、SCC患者血清中的表达为（324.71±75.87）、（397.43±68.56）、（482.77±104.05）pg/ml；IL-18在CINⅠ期、CINⅡ～Ⅲ期、SCC患者血清中的表达分别为（305.53±64.08）、（392.74±87.38）、（499.86±92.04）pg/ml，差异均有统计学意义（F=17.657，P=0.001；F=13.309，P=0.003）。IL-10在正常对照组、CINⅠ期、CINⅡ～Ⅲ期、SCC患者组织中的相对表达量分别为0.99±0.01、3.24±0.68、7.32±0.99、13.24±1.03；IL-18在正常对照组、CINⅠ期、CINⅡ～Ⅲ期、SCC患者组织中的相对表达量分别为0.98±0.01、2.02±0.84、7.01±1.59、14.38±2.10；差异均有统计学意义（F=21.694，P=0.000；F=19.912，P=0.001）。IL-10、IL-18的表达与HPV的感染呈正相关（r=0.696，P=0.001；r=0.852，P=0.001）。高表达IL10的患者其中位生存时间为9.74个月，较低表达者（24.47个月）明显缩短，差异有统计学意义（χ2=21.363，P=0.001）；高表达IL-18患者的中位生存时间为12.32个月，较低表达者（22.88个月）明显缩短，差异有统计学意义（χ2=7.457，P=0.006）。结论 IL-10和IL-18在宫颈癌前病变及宫颈癌血清、组织中呈高表达，可作为早期预测宫颈病变的生物标志物。

关键词: 白细胞介素类, 宫颈肿瘤, 癌前状态

Abstract: Objective To explore the expressions and significancse of interleukin (IL)-10 and IL-18 in serum and tissues of patients with cervical intraepithelial neoplasias (CIN) and cervical cancer, and to analyze their relationships with clinical pathological characteristics and prognosis. Methods One hundred and eight patients′ tissues and clinical blood samples (68 cases of CIN and 40 cases of squamous cell carcinoma) were enrolled in this study, including 25 with low-grade cervical intraepithelial neoplasias (CINⅠ), 43 with high-grade cervical intraepithelial neoplasias (CINⅡ-Ⅲ), and 40 with invasive cervical squamous cell carcinoma (SCC); meanwhile, 20 cases normal cervical tissues were enrolled. The expressions of IL-10 and IL-18 in cervical tissues and serum of the studied patients were measured by enzyme-linked immunosorbent assay and polymerase chain reaction. Meanwhile, second generation hybrid capture was used to detect human papilloma virus(HPV) DNA in cervical smears obtained from the studied patients with the different lesions. The relationships among IL-10 and IL-18 with HPV DNA and clinical prognosis were analyzed. Results The expressions of IL-10 and IL-18 in normal control serum were (212.75±62.09), (187.84±81.03)pg/ml respectively. The expressions of IL-10 in CINⅠ, CINⅡ-Ⅲ and SCC serums were (324.71±75.87), (397.43±68.56), (482.77±104.05)pg/ml, with statistically significant difference (F=17.657, P=0.001). The expressions of IL-18 in CINⅠ, CINⅡ-Ⅲ and SCC serums were (305.53±64.08), (392.74±87.38), (499.86±92.04)pg/ml, with statistically significant difference (F=13.309, P=0.003).The relative expressions of IL-10 in normal control, CINⅠ, CINⅡ-Ⅲ and SCC tissues were 0.99±0.01, 3.24±0.68, 7.32±0.99, 13.24±1.03, with statistically significant difference (F=21.694, P=0.000). The relative expressions of IL-18 in normal control, CINⅠ, CINⅡ-Ⅲ and SCC tissues were 0.98±0.01, 2.02±0.84, 7.01±1.59, 14.38±2.10, with statistically significant difference (F=19.912， P=0.001). The expressions of IL-10 and IL-18 were positively associated with HPV infection (r= 0.696, P=0.001; r=0.852, P=0.001). The median survival time of patients with high expressions of IL-10 was 9.74 months, which obviously shorter than those patients with low expressions of IL-10 (24.47 months), with statistically significant difference (χ2=21.363, P=0.001). The median survival time of patients with high expressions of IL-18 was 12.32 months, which obviously shorter than those patients with low expressions of IL-18 (22.88 months), with statistically significant difference (χ2=7.457, P=0.006). Conclusion High expressions of IL-10 and IL-18 are observed in patients with CIN and SCC, which can be used as biomarkers for predicting early cervical lesions.

Key words: Interleukins, Uterine cervical neoplasms, Precancerous conditions