国际肿瘤学杂志

国际肿瘤学杂志 ›› 2013, Vol. 40 ›› Issue (6): 476-479.

• 论著 • 上一篇    下一篇

microRNA-200b对人结肠癌细胞增殖和迁移的影响

杜叶平, 武春梅, 房树志, 吴洁, 苗晋华   

  1. 030001太原，解放军第二六四医院检验科（杜叶平、苗晋华、武春梅、吴洁），医院办公室（房树志）

  • 出版日期:2013-06-08 发布日期:2013-05-24
  • 通讯作者: 苗晋华，E-mail：miaojh337@163.com E-mail:miaojh337@163.com

Effects of microRNA-200b on proliferation and migration of colon cancer cells

DU  Ye-Ping, WU  Chun-Mei, FANG  Shu-Zhi, WU  Jie, MIAO  Jin-Hua-   

  1. Department of Laboratory, 264 Hospital of PLA, Taiyuan 030001, China

  • Online:2013-06-08 Published:2013-05-24
  • Contact: MIAO Jin-Hua E-mail:miaojh337@163.com

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摘要： 目的  研究miR-200b对人结肠癌sw620细胞E-cadherin表达的调节作用，探讨miR-200b对sw620细胞增殖及迁移的影响。方法  采用RT-PCR技术分别检测转染pEGP-miR-200b后24 h和72 h sw620细胞中miR-200b的表达变化；采用RT-PCR技术和Western blot方法检测转染miR-200b后对E-cadherin表达的影响；采用四甲基偶氮唑蓝法（MTT）和划痕擦伤实验检测转染miR-200b对sw620细胞增殖、迁移的影响。结果  miR-200b转染sw620细胞48 h和72 h后，miR-200b相对表达量较对照组显著上调(t=11.579，P＜0.01；t=11.579，P＜0.01)；且抑制了细胞的增殖能力，第3天抑制作用最显著，抑制率为55.34%；细胞的迁移速度受到明显的抑制，两组在24、48、72 h的迁移距离有显著差异（t=11.579，P＜0.01；t=10.419，P＜0.01；t=6.955，P＜0.01）。同时细胞中E-cadherin基因的mRNA和蛋白表达水平显著升高（t=10.432，P＜0.01；t=8.325，P＜0.01）。结论  上调miR-200b表达可抑制结肠癌细胞的生物活性，该作用可能与E-cadherin的表达有关。

关键词: 微RNAs, 结肠肿瘤, 细胞增殖, 细胞迁移分析

Abstract: Objective  To study the effects of miR-200b on proliferation and migration of sw620 colon cancer cells, and its regulation effect on E-cadherin expression. Methods  The expressions of miR-200b in sw620 cells at 24 h and 72 h after pEGP-miR-200b transfection were detected by real-time PCR (RT-PCR). The change of the expression level of E-cadherin after miR-200b transfection was detected using the methods of RT-PCR and Western blot. The proliferation and migration abilities were measured by MTT and scratch test after miR-200b transfection. Results  The expressions of miR-200b in sw620 cells at 24 h and 72 h after pEGP-miR-200b transfection raised significantly compared to the control group (t=11.579, P＜0.01; t=11.579, P＜0.01). MiR-200b transfection inhibited the proliferation abilities of sw620 cells. It is the most significant of the inhibitory effect on the third day and the inhibition rate was 55.34%. MiR-200b transfection  markedly inhibited the migration abilities of sw620 cells. The two groups had significant difference in the migration distance of 24, 48, 72 h (t=11.579, P＜0.01; t=10.419, P＜0.01; t=6.955, P＜0.01). The mRNA and protein expressions of E-cadherin gene increased significantly by transfecting miR-200b gene in sw620 cells (t=10.432, P＜0.01; t=8.325, P＜0.01). Conclusion  Up-regulated expression of miR-200b could inhibite the proliferation and migration abilities of sw620 colon cancer cells. The involved molecular mechanism is probably related to the change of E-cadherin expression.

Key words: MicroRNAs, Colonic neoplasms, Cell proliferation, Cell migration assays