Ribosomal protein S7 affects apoptosis of HeLa cervical cancer cells

doi:10.3760/cma.j.issn.1673422X.2015.08.007

Abstract

Abstract: ObjectiveTo preliminarily investigate the effect of ribosomal protein S7 on apoptosis of HeLa cervical cancer cells. MethodsThe previously constructed recombinant plasmid pIRES2EGFPRPS7 was transfected into HeLa cells, the empty vector pIRES2EGFP transfected cells as control. Enhanced green fluorescent protein(EGFP) expressing cells were quantified by flow cytometry, and RPS7 protein level was also determined by Western blotting. Cell apoptosis of both RPS7 overexpression cells and knockdown cells were evaluated by flow cytometry after staining using allophycocyanin labeled AnnexinV. ResultsApoptotic cell level in the obtained RPS7 transient overexpression HeLa cells was significantly higher than that of vector control cells ［(10.00±0.60)% vs (5.73±0.61)%］， with a statistic difference (t=8.63, P = 0.001). Moreover, the apoptotic level in RPS7 knockdown cells was lower than that in control cells ［(3.08±0.49)% vs (5.97±0.63)%］, with a statistic difference (t=6.40, P=0.003). ConclusionUpregulation of RPS7 may promote apoptosis, while downregulation of RPS7 may inhibit apoptosis of HeLa cells, indicating that RPS7 may play roles in regulating cell apoptosis.

Key words: Uterine cervical neoplasms, Apoptosis, Ribosomal protein S7

DING Hui, LING Jing-Xian, CHEN Jun-Hao, ZHANG Kui, WANG Qing-Fei. Ribosomal protein S7 affects apoptosis of HeLa cervical cancer cells[J]. Journal of International Oncology, 2015, 42(8): 585-588.

References

［1］ Uechi T, Tanaka T, Kenmochi N. A complete map of the human ribosomal protein genes: assignment of 80 genes to the cytogenetic map and implications for human disorders［J］. Genomics, 2001, 72(3):223230. ［2］ Warner JR, McIntosh KB. How common are extraribosomal functions of ribosomal proteins?［J］. Mol Cell, 2009, 34(1):311. ［3］王庆飞，丁慧，刘宝瑞, 等. 核糖体蛋白S7真核表达载体的构建及其对293细胞凋亡的影响［J］. 中华实验外科杂志, 2013, 30(11):2464. ［4］丁慧，禹立霞，凌静娴，等. 稳定干扰核糖体蛋白RPS7基因表达的宫颈癌HeLa细胞株的建立［J］. 医学分子生物学杂志, 2013, 10(6):318322. ［5］ Lindstrm MS. Emerging functions of ribosomal proteins in genespecific transcription and translation［J］. Biochem Biophys Res Commun, 2009, 379(2):167170. ［6］ Ruggero D, Pandolfi PP. Does the ribosome translate cancer?［J］. Nat Rev Cancer, 2003, 3(3):179192. ［7］ Wang Z, Hou J, Lu L, et al. Small ribosomal protein subunit S7 suppresses ovarian tumorigenesis through regulation of the PI3K/AKT and MAPK pathways［J］. PLoS One, 2013, 8(11): e79117. ［8］ Chen D, Zhang Z, Li M, et al. Ribosomal protein S7 as a novel modulator of p53MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function［J］. Oncogene, 2007, 26(35):50295037. ［9］ Zhu Y, Poyurovsky MV, Li Y, et al. Ribosomal protein S7 is both a regulator and a substrate of MDM2［J］. Mol Cell, 2009, 35(3):316326. ［10］ Zhang Y, Lu H. Signaling to p53: ribosomal proteins find their way［J］. Cancer Cell, 2009, 16(5):369377. ［11］ Gao M, Li X, Dong W, et al. Ribosomal protein S7 regulates arseniteinduced GADD45alpha expression by attenuating MDM2mediated GADD45alpha ubiquitination and degradation ［J］. Nucleic Acids Res, 2013, 41(10):52105222. ［12］ Duan J, Ba Q, Wang Z, et al. Knockdown of ribosomal protein S7 causes developmental abnormalities via p53 dependent and independent pathways in zebrafish［J］. Int J Biochem Cell Biol, 2011, 43(8):12181227.

[1]	Zhang Ningning, Yang Zhe, Tan Limei, Li Zhenning, Wang Di, Wei Yongzhi. Diagnostic value of cervical cell DNA ploidy analysis combined with B7-H4 and PKCδ for cervical cancer [J]. Journal of International Oncology, 2024, 51(5): 286-291.
[2]	Ren Lu, Xie Xiaoli, Zhang Kun, Wang Lijuan. Effects and mechanisms of dihydroartemisinin combined with carfilzomib on the activity， proliferation， and apoptosis of multiple myeloma cells [J]. Journal of International Oncology, 2024, 51(3): 129-136.
[3]	Zhang Keping, Zhao Yongsheng, Yang Juan, Fu Maoyong. Chlorogenic acid induces mitochondrial dysfunction in lung cancer A549 cells by inhibiting the PI3K-Akt pathway [J]. Journal of International Oncology, 2024, 51(1): 21-28.
[4]	Xiang Yuling, Tan Jiajie, Xiong Yuanguo, Zhao Lirong, Li Chen, Zhang Hong. Effects of Anhydroicaritin on the proliferation， migration and apoptosis of hepatocellular carcinoma cells [J]. Journal of International Oncology, 2023, 50(9): 513-519.
[5]	Feng Dongxu, Wu Wei, Gao Pingfa, Wang Jun, Shi Lijuan, Chen Dawei, Li Wenbing, Zhang Meifeng. Effects of miR-451 on glycolysis and apoptosis of breast cancer cells by regulating Rho/ROCK1 pathway [J]. Journal of International Oncology, 2023, 50(8): 449-456.
[6]	Zhang Lu, Jiang Hua, Lin Zhou, Ma Chenying, Xu Xiaoting, Wang Lili, Zhou Juying. Analysis of curative effect and prognosis of immune checkpoint inhibitor in the treatment of recurrent and metastatic cervical cancer [J]. Journal of International Oncology, 2023, 50(8): 475-483.
[7]	Wu Jiali, Zhang Jiahui, Zhang Ping, Xiao Xinyue, Li Rui, Zhang Hongyu. Mechanism of Bcl-2 BH4 selective inhibitor BDA-366 on NK/T cell lymphoma cells [J]. Journal of International Oncology, 2023, 50(7): 413-418.
[8]	Lyu Lu, Sun Pengfei. Gut flora and cervical cancer [J]. Journal of International Oncology, 2023, 50(6): 373-376.
[9]	Yang Ya, Wang Huili, Liu Yan, Guo Jinfeng, Wang Chunxia, Lyu Min, Shan Changping. Effects of GCSH gene on proliferation and apoptosis of gastric cancer SNU-1 cells [J]. Journal of International Oncology, 2023, 50(5): 257-262.
[10]	Zhu Yi, Chen Jian. Mechanism of hydrogen sulfide in tumorigenesis and development and its donor-mediated anti-tumor effects [J]. Journal of International Oncology, 2023, 50(12): 729-733.
[11]	Ma Xueyan, Lu Lili, Sun Pengfei. Advances in the immune microenvironment in cervical cancer [J]. Journal of International Oncology, 2023, 50(1): 47-50.
[12]	Zhang Lu, Zhou Juying, Ma Chenying, Lin Zhou. Advances in immunotherapy for recurrent and metastatic cervical cancer [J]. Journal of International Oncology, 2022, 49(9): 517-520.
[13]	Shi Yingxia, Hu Lijun, Yu Jingping. Application of immune checkpoint inhibitors in the treatment of recurrent or metastatic cervical cancer [J]. Journal of International Oncology, 2022, 49(9): 568-571.
[14]	Peng Chen, Xie Yintong, Zhang Xin, Xie Peng. Research progress of maintenance therapy for cervical cancer [J]. Journal of International Oncology, 2022, 49(7): 430-435.
[15]	Lian Haiwei, Yang Shuorui, Liu Renzhong. Mechanism study on regulation of glioblastoma cell proliferation and apoptosis by sciadopitysin combined with CX-4945 through Notch1 pathway [J]. Journal of International Oncology, 2022, 49(6): 321-326.