Abstract: There are many ways to identify circulating tumor cells in the current. Fluorescence has a wide range of applications in the identification of circulating tumor cells. The labeled cells can be observed and counted more intuitively by labeling the tumor cells with fluorescent group containing antibodies, probes and aptamers, and cytokine, CD45 and fluorescence in situ hybridization are widely used in the identification of various circulating tumor cell related tests. In recent years, people have explored the feasibility of using fluorescent probes to directly identify circulating tumor cells. With the development of biopsy probes and optical sectioning imaging technology of confocal microscopy, it is possible to directly identify circulating tumor cells with fluorescent probes in the future.

Key words: Neoplasm circulating cells, Fluorescence, In situ hybridization, fluorescence, Probe