国际肿瘤学杂志

国际肿瘤学杂志 ›› 2017, Vol. 44 ›› Issue (1): 11-14.doi: 10.3760/cma.j.issn.1673-422X.2017.01.003

• 论著 • 上一篇    下一篇

AEG-1与BECN-1在甲状腺乳头状癌中的表达及其临床意义

任佳,宋宁宁,王新超   

  1. 300193 天津中医药大学研究生院（任佳）；天津医科大学研究生院（宋宁宁）；天津市第四中心医院乳腺甲状腺外科（王新超）
  • 出版日期:2017-01-08 发布日期:2016-12-07
  • 通讯作者: 王新超，Email: 925293202@qq.com E-mail:925293202@qq.com

Expressions and clinical significance of AEG-1 and BECN-1 in papillary thyroid carcinoma

Ren Jia, Song Ningning, Wang Xinchao   

  1. Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Online:2017-01-08 Published:2016-12-07
  • Contact: Wang Xinchao, Email: 925293202@qq.com E-mail:925293202@qq.com

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1

摘要： 目的  探讨甲状腺乳头状癌(PTC)组织中星形细胞上调基因-1（AEG-1）与Beclin-1基因（BECN-1）的表达及其临床意义。方法  选取2015年10月至2016年5月于天津医科大学总医院行甲状腺全切术的30例PTC患者的组织标本，患者均为单侧发病。其中PTC组织取自患侧甲状腺，正常组织取自对侧正常甲状腺。采用反转录聚合酶链反应（RT-PCR）法检测AEG-1与BECN-1在PTC中的表达水平，并分析两者在PTC中的表达与患者临床病理特征的关系。 结果  AEG-1在PTC和正常组织中的mRNA测得值分别为0.626 1（0.741 4）和0.049 8（0.011  8），差异有统计学意义（Z=-4.488,P=0.000）。BECN-1在PTC和正常组织中的mRNA测得值分别为0.067 9（0.198 1）和0.785 7（0.361 7），差异有统计学意义（Z=-5.441,P=0.000）。在PTC中，AEG-1和BECN-1的表达与TNM分期（Z=-3.980,P=0.000；Z=-2.265,P=0.023）、淋巴结转移（Z=-3.190,P= 0.001；Z=-2.640,P=0.008）相关，但与发病年龄（Z=-1.203,P=0.229；Z=-1.162,P=0.245）、性别（Z=-1.222,P=0.222；Z=-0.453,P=0.651）、肿瘤大小（Z=-1.496,P=0.135；Z=-1.166,P=0.244）无关。在PTC中AEG-1与BECN-1的表达呈负相关（r=-0.343,P=0.007）。结论  AEG-1在PTC组织中的表达高于正常组织，BECN-1在PTC组织中的表达低于正常组织，AEG-1和BECN1的表达与TNM分期和有无淋巴结转移相关，可作为PTC预后评价的参考指标。

关键词: 甲状腺肿瘤, 星形细胞上调基因-1, Beclin-1基因

Abstract: Objective  To analyze the expressions of astrocyte elevated gene-1 (AEG-1) and Beclin-1 (BECN-1) in papillary thyroid carcinoma （PTC） and their clinical significances. Methods  Thirty patients with PTC who were performed total thyroidectomy in the General Hospital Affiliated to Tianjin Medical University from October 2015 to May 2016 were collected. All patients were diagnosed with unilateral PTC. The tumor tissues were taken from the patients′ ipsilateral thyroids, and 30 cases of normal tissues were taken from the patients′ contralateral normal thyroids. The expressions of AEG-1 and BECN-1 in PTC tissues were detected by reverse transcriptionpolymerase chain reaction (RT-PCR). The relationships between the expression status and clinicopathologic features were analyzed. Results  The positive expression levels ［M(QR)］ of AEG-1 in PTC and the normal tissues were 0.626 1 （0.741 4） and 0.049 8 （0.011 8） respectively, with a significant difference (Z=-4.488, P=0.000). The positive expression levels of BECN-1 in PTC and the normal tissues were 0.067 9 （0.198 1） and 0.785 7 （0.361 7） respectively, with a significant difference (Z=-5.441, P=0.000). In PTC, the expressions of AEG-1 and BECN-1 were related to TNM staging (Z=-3.980, P=0.000; Z=-2.265, P=0.023) and lymphatic metastasis (Z=-3.190, P=0.001; Z=-2.640, P=0.008), but they were not related to age (Z=-1.203, P=0.229; Z=-1.162, P=0.245), gender (Z=-1.222, P=0.222; Z=-0.453, P=0.651) and tumor size (Z=-1.496, P=0.135; Z=-1.166, P=0.244). The expression of AEG-1 was negatively correlated with that of BECN-1 (r=-0.343, P=0.007).Conclusion  The expression of AEG-1 in PTC is higher than that in normal tissues and the expression of BECN-1 in PTC is lower than that in normal tissues， and the expressions of AEG-1 and BECN-1 are related to TNM staging and lymphatic metastasis, which are expected to become prognostic indicators.

Key words: Thyroid neoplasms, Astrocyte elevated gene-1, Beclin-1 gene